亚洲视频一区二区三区四区-亚洲视频一区二区三区-亚洲视频一区二区-亚洲视频一区-亚洲视频一二三-亚洲视频一二区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>NCI-H226細(xì)胞, 人肺癌細(xì)胞株

NCI-H226細(xì)胞, 人肺癌細(xì)胞株

簡要描述:NCI-H226細(xì)胞, 人肺癌細(xì)胞株
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:1970

產(chǎn)品分類

Product Category

詳細(xì)介紹

NCI-H226細(xì)胞, 人肺癌細(xì)胞株

ATCC® Number: CRL-5826™
Designations: NCI-H226 [H226]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: Epithelial

NCI-H226細(xì)胞, 人肺癌細(xì)胞株
Source: Organ: lung
Disease: squamous cell carcinoma; mesothelioma [23570]
Derived from metastatic site: pleural effusion
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.
Isolation: Isolation date: March, 1980
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 13,14
D16S539: 9,12
D5S818: 11,12
D7S820: 8,10
THO1: 8,9.3
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25)
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37C.

Subc*tion Ratio: 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to3 days
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 欧美一区精品二区三区| 国产三级精品播放| japanese厕所撒尿| 四虎在线视频免费观看| 农村脱精光一级| 久久精品嫩草影院免费看| 亚洲男人天堂网站| japanesexxxx日本妞| 古装一级无遮挡毛片免费观看| 日本黄色一区| sxx免费看观看美女| 亚洲国产精品婷婷久久久久| 69av美女| 亚洲国产免费观看视频| www.久久艹| 日本一区二区免费在线| 动漫美女被吸乳羞羞小说| 午夜香蕉| 久久综合网久久综合| 日本人护士免费xxxx视频| 99热r| 日本天堂影院在线播放| 973影院| darkside动漫在线观看| 亚洲爱v| 丝瓜视频成人在线观看| 国产精品微拍| 脱了白丝校花的内裤猛烈进入| 美女乳液| 九九99热| 日日摸日日碰夜夜爽97纠| 国产ay| 日韩欧美色图| 精品在线一区| 精品视频二区| 999久久精品国产| 色热综合| 韩国伦理hd| 99视频在线免费观看| 91麻豆在线观看| 国产一区二区三区久久精品小说|