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MMQ 大鼠垂體瘤細胞

簡要描述:CRL-10609 MMQ 大鼠垂體瘤細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件

  • 產品型號:CRL-10609
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  • 更新時間:2024-11-14
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CRL-10609 MMQ 大鼠垂體瘤細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻優培養條件


CRL-10609 MMQ 大鼠垂體瘤細胞 的詳細介紹

CRL-10609

ATCC® Number: CRL-10609™    Price: $438.00
Designations: MMQ

Depositors: University of Virginia Patent Foundation

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:suspension

Organism:Rattus norvegicus (rat)

Morphology:epithelial


Source:Organ: pituitary
Disease: tumor


Cellular Products:prolactin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors:dopamine

Tumorigenic:No

Age: 5 days

Comments:The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 2.5%

  • horse serum to a final concentration of 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    DGrowth Conditions: The line grows as clumps of cells in suspension.



Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 days


Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Doubling Time: 24 to 48 hrs

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040

Cell culture tested DMSO:ATCC 4-X



References:22090: MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996
























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